In molecular biology, the stretch of nucleotides of DNA that lie in the 3’ direction from the site of initiation of transcription, which is designated as +1 (remembering the convention that the sequence of a DNA molecule is written from the 5’ end to the 3’ end). Downstream nucleotides are marked with plus signs, e.g., +2, +10. Also, to the 3’ side of a particular gene or sequence of nucleotides.